mfagan

 

BIOL 361

Page history last edited by Anonymous 3 yrs ago
  • basics
    • sample vs. population
      • in experiment, the data is the sample, all possible experiments is the population
      • in bio, usually population is infinite/big relative to sample
    • statistical modeling: how well experiment fits math model (eg linear regression - best fit line)
    • descriptive stats (describe study/experiment) vs. inferential stats (extrapolate to pop. eg hypothesis testing, stats modeling)
    • variable is the property being measured
    • data types: quantitative (continuous vs. discrete) vs qualitative
      • 4 levels in reverse hierarchy: nominal (qual, no order), ordinal (qual, no ratio), interval (quan, no zero-point), ratio (quan)
    • accuracy (close to truth) vs. precision (close to each other)
      • good precision to use: between range/30 & range/300
    • derived variable: function(2+ vars) eg ratio

 

  • hypothesis testing
    • hypthesis: rejected or not rejected; divide into null (H0) and alternative (H1)
    • statistical test: p=probability(H0=true)
    • errors: Type 1 (reject H0 when true), Type 2 (don't reject H0 when false)
      • p(T1 occurring) = α (usually 0.05)     p(T2) = β     α, β, and n all related

 

  • experiments: mensurative vs. manipulative
    • manipulative control: procedural (non-manipulated group for comparison), temporal (eg measure before & after)
    • statistical control: measure factor instead of fixing it

 


 

 

  • nominal, ordinal, interval, ratio
  • Type 1: reject H0
    • α T1 rate
  • variable=property
  • precision=close together
  • p p(H0=true)
  • CV = SD as % of mean (compare SDs even w/ diff means)
  • skew g1; kurtosis g2
  • CI range (around μ), w/ d.confidence (1-α; that % of CIs would contain true value)
    • need μ, variability, n, d.c
    • assume random (independent & from same pop.) sample, normal
  • t-dist assume pop. normal; d.f=n-1 less flat (more normal) w/ n (n>30 approx normal)
  • χ2 to estimate SD (vs z/t for μ & proportions) d.f=n-1
  • H0 always includes =; p<=α is significant
  • critical region: tail(s) where reject H0
  • parametric = assumed normal
  • test μ 1-sample test (p or CI (adjust if 1-tailed))
  • compare 2 μs
    • paired (d.f=pairs-1, assume duh) parametric (paired t-test (use difference)), non-parametric (Wilcoxon Signed Ranks H0:medians =)
    • unpaired (d.f=Σ(n-1)) parametric (independent t-test (pooled if assume =SD, n<=30)), non-parametric (Mann-Whitey U, W.R sum)
  • comparing SD2
      if normal Leven's test (1 is 1 w/ > variance) d.f=d.f 1/2
  • test normality
    • graphs: histogram, normal quantile (Q-Q; n<50 use Rankits), normal prob (P-P)
    • formal: Kolmogorov-Smirnov (unknown μ), Shapero-Wilk (n<50), skew, kurtosis, g test, chi2 test
  • ANOVA (>2 μs) d.f=groups-1,groups×(n-1) (assume normal, =variances, independent; 1 diff factor; 1st,2nd assumptions relaxed in 1-way)
    • sum of squared: variance before ÷n-1; MS(mean squares) = SS/d.f
    • F MStreatment/MSerror (>F = more group diff; F=1 is = means)
    • Kruskal-Wallis (K in SPSS)= non-parametric equivalent (assume independent, same distribution shapes)
    • post-hoc: share α among comparisons

 

lec 7 includes testing sample proportions...

 

 

r2 is fraction of variance that's shared

aka % of one due to the other

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